| Primary Identifier | MGI:4833641 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Serpina6 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| description | Gene targeting to generate Serpina6tm1Mmp was performed at the Mouse Clinical Institute (Illkirch, France)(J:164345), which according to their Web site (http://www.ics-mci.fr/index.html) employs "ES cells of the 129SvPas strain." |
| molecularNote | The loxP-flanked exon 2 of Serpina6tm1Mmp, containing the entire protein coding sequence, has been excised by Cre recombinase. Quantitative real-time PCR analysis of liver mRNA detects no transcripts from the targeted gene in homozygous mutant mice and ~50% of wild-type transcript levels in heterozygous mice. Immunoblot analysis of plasma of homozygous mutant, heterozygous and wild-type mice yields confirmatory results for the for the protein. |
