| Primary Identifier | MGI:4834648 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| description | Germ line transmission of mutant cell line mEMS2784 has been established. |
| molecularNote | The Ple67-EGFP transgene (pEMS1401) was designed with the 2242 bp Ple67 minipromoter (FEV-D; derived from a subsection of the promoter from the human FEV (ETS oncogene family) (FEV) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human FEV gene directs expression of enhanced green fluorescent protein (EGFP) to neuronal cell bodies with strong staining in the nucleus. All raphe nuclei show EGFP positive cells: dorsal raphe, obscurus/magnus raphe and pallidus raphe. |
