| Primary Identifier | MGI:4834790 | Allele Type | Targeted |
| Gene | Hprt1 | Transmission | Germline |
| Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 | Is Recombinase | false |
| Is Wild Type | false | Project Collection | Pleiades Promoter Project |
| description | Germ line transmission of mutant cell line mEMS4135 has been established. |
| molecularNote | The Ple160-lacZ transgene (pEMS1631) was designed with the 2538 bp Ple160 minipromoter (PITX3-B; derived from a subsection of the promoter from the human paired-like homeodomain 3 (PITX3) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human paired-like homeodomain 3 (PITX3) gene directs expression of lacZ to anterior thalamus, with further expression observed in the ventral-lateral hippocampus, and the VTA region. The latter (VTA region) location reflects the expression of the endogenous Pitx3 gene. |
