| First Author | Saintamand A | Year | 2017 |
| Journal | Sci Rep | Volume | 7 |
| Issue | 1 | Pages | 442 |
| PubMed ID | 28348365 | Mgi Jnum | J:333215 |
| Mgi Id | MGI:6726707 | Doi | 10.1038/s41598-017-00575-0 |
| Citation | Saintamand A, et al. (2017) Emu and 3'RR IgH enhancers show hierarchic unilateral dependence in mature B-cells. Sci Rep 7(1):442 |
| abstractText | Enhancer and super-enhancers are master regulators of cell fate. While they act at long-distances on adjacent genes, it is unclear whether they also act on one another. The immunoglobulin heavy chain (IgH) locus is unique in carrying two super-enhancers at both ends of the constant gene cluster: the 5'Emu super-enhancer promotes VDJ recombination during the earliest steps of B-cell ontogeny while the 3' regulatory region (3'RR) is essential for late differentiation. Since they carry functional synergies in mature B-cells and physically interact during IgH locus DNA looping, we investigated if they were independent engines of locus remodelling or if their function was more intimately intermingled, their optimal activation then requiring physical contact with each other. Analysis of chromatin marks, enhancer RNA transcription and accessibility in Emu- and 3'RR-deficient mice show, in mature activated B-cells, an unilateral dependence of this pair of enhancers: while the 3'RR acts in autonomy, Emu in contrast likely falls under control of the 3'RR. |
