| First Author | Hozumi Y | Year | 2015 |
| Journal | Brain Res | Volume | 1594 |
| Pages | 36-45 | PubMed ID | 25446448 |
| Mgi Jnum | J:218593 | Mgi Id | MGI:5618025 |
| Doi | 10.1016/j.brainres.2014.11.012 | Citation | Hozumi Y, et al. (2015) Involvement of diacylglycerol kinase beta in the spine formation at distal dendrites of striatal medium spiny neurons. Brain Res 1594:36-45 |
| abstractText | Spine formation, a salient feature underlying neuronal plasticity to adapt to a changing environment, is regulated by complex machinery involving membrane signal transduction. The diacylglycerol kinase (DGK) family, which is involved in membrane lipid metabolism, catalyzes the phosphorylation of a lipid second messenger, diacylglycerol (DG). Of the DGKs, DGKbeta is characterized by predominant expression in a specific brain region: the striatum. We previously demonstrated that DGKbeta is expressed selectively in medium spiny neurons (MSNs) and that it is highly enriched in the perisynaptic membrane on dendritic spines contacted with excitatory terminals. Moreover, DGKbeta regulates spinogenesis through actin-based remodeling in an activity-dependent manner. However, the detailed mechanisms of spinogenesis regulation and its functional significance remain unclear. To address these issues, we performed Golgi-Cox staining to examine morphological aspects of MSNs in the striatum of DGKbeta-knockout (KO) mice. Results show that striatal MSNs of DGKbeta-KO mice exhibited lower dendritic spine density at distal dendrites than wild-type mice did. We also sought protein targets that interact with DGKbeta and identified the GluA2 AMPA receptor subunit as a novel DGKbeta binding partner. In addition, DGKbeta-deficient brain exhibits significant reduction of TARP gamma-8, which represents a transmembrane AMPA receptor regulatory protein. These findings suggest that DGKbeta regulates the spine formation at distal dendrites in MSNs. |
