| First Author | Fukuoka M | Year | 2023 |
| Journal | Int J Mol Sci | Volume | 25 |
| Issue | 1 | PubMed ID | 38203456 |
| Mgi Jnum | J:351169 | Mgi Id | MGI:7573524 |
| Doi | 10.3390/ijms25010285 | Citation | Fukuoka M, et al. (2023) Overdue Calcium Oscillation Causes Polyspermy but Possibly Permits Normal Development in Mouse Eggs. Int J Mol Sci 25(1) |
| abstractText | In some non-mammalian eggs, the fusion of one egg and multiple sperm (polyspermy) induces a robust rise in intracellular calcium ion (Ca(2+)) concentration due to a shortage of inducers carried by a single sperm. Instead, one of the sperm nuclei is selected inside the egg for normal embryogenesis. Polyspermy also occurs during the in vitro fertilization of human eggs; however, the fate of such eggs is still under debate. Hence, the relationship between polyspermy and repetitive Ca(2+) increases (Ca(2+) oscillation) in mammals remains unknown. To address this issue, we used mouse sperm lacking extramitochondrial citrate synthase (eCS), which functions as a Ca(2+) oscillation inducer; its lack causes retarded Ca(2+) oscillation initiation (eCs-KO sperm). Elevated sperm concentrations normalize Ca(2+) oscillation initiation. As expected, eCS deficiency enhanced polyspermy in both zona pellucida (ZP)-free and ZP-intact eggs despite producing the next generation of eCs-KO males. In conclusion, similarly to non-mammalian eggs, mouse eggs may develop normally under polyspermy conditions caused by problematic Ca(2+) oscillation. |
