| Primary Identifier | MGI:4843449 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Ptcra |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neomycin resistance cassette in reverse orientation was inserted in intron 1. Mutations were inserted in exon 2 causing an aspartic acid to alanine change at codon 22 (D22A) and arginine to alanine changes at codons 24 and 102 (R24A, R102A). An additional mutation in exon 3 changes codon 117 from arginine to alanine (R117A). All four mutated amino acids are in the extracellular domain of the protein. The selection cassette was subsequently removed by cre excision. |
