| First Author | Nomura S | Year | 2020 |
| Journal | iScience | Volume | 23 |
| Issue | 11 | Pages | 101710 |
| PubMed ID | 33196030 | Mgi Jnum | J:349761 |
| Mgi Id | MGI:6717528 | Doi | 10.1016/j.isci.2020.101710 |
| Citation | Nomura S, et al. (2020) Combined Optogenetic Approaches Reveal Quantitative Dynamics of Endogenous Noradrenergic Transmission in the Brain. iScience 23(11):101710 |
| abstractText | Little is known about the real-time cellular dynamics triggered by endogenous catecholamine release despite their importance in brain functions. To address this issue, we expressed channelrhodopsin in locus coeruleus neurons and protein kinase-A activity biosensors in cortical pyramidal neurons and combined two-photon imaging of biosensors with photostimulation of locus coeruleus cortical axons, in acute slices and in vivo. Burst photostimulation of axons for 5-10 s elicited robust, minutes-lasting kinase-A activation in individual neurons, indicating that a single burst firing episode of synchronized locus coeruleus neurons has rapid and lasting effects on cortical network. Responses were mediated by beta1 adrenoceptors, dampened by co-activation of alpha2 adrenoceptors, and dramatically increased upon inhibition of noradrenaline reuptake transporter. Dopamine receptors were not involved, showing that kinase-A activation was due to noradrenaline release. Our study shows that noradrenergic transmission can be characterized with high spatiotemporal resolution in brain slices and in vivo using optogenetic tools. |
