| First Author | Berger A | Year | 2010 |
| Journal | Blood | Volume | 116 |
| Issue | 19 | Pages | 3792-801 |
| PubMed ID | 20660792 | Mgi Jnum | J:166471 |
| Mgi Id | MGI:4845815 | Doi | 10.1182/blood-2010-06-291062 |
| Citation | Berger A, et al. (2010) Targeted deletion of the tachykinin 4 gene (TAC4-/-) influences the early stages of B lymphocyte development. Blood 116(19):3792-801 |
| abstractText | Hemokinin-1 (HK-1), encoded by the TAC4 gene, is a tachykinin peptide that is predominantly expressed in non-neuronal cells, such as immune cells. We have disrupted the mouse TAC4 gene to obtain a better understanding of the actions of HK-1 during hematopoiesis. We demonstrate here that TAC4(-/-) mice exhibit an increase of CD19(+)CD117(+)HSA(+)BP.1(-) 'fraction B' pro-B cells in the bone marrow, whereas pre-B, immature, and mature B cells are within the normal range. We show that in vitro cultures derived from TAC4(-/-) bone marrow, sorted 'fraction B' pro-B cells or purified long-term reconstituting stem cells, contain significantly higher numbers of pro-B cells compared with controls, suggesting an inhibitory role for HK-1 on developing B cells. Supporting this idea, we show that addition of HK-1 to cultures established from long-term reconstituting stem cells and the newly described intermediate-term reconstituting stem cells leads to a significant decrease of de novo generated pro-B cells. Based on our studies, we postulate that HK-1 plays an inhibitory role in hematopoiesis, and we hypothesize that it may be part of the bone marrow microenvironment that supports and regulates the proliferation and differentiation of hematopoietic cells. |
