| Primary Identifier | MGI:4867475 | Allele Type | Targeted |
| Attribute String | Dominant negative, Inserted expressed sequence, Null/knockout | Gene | Mesp2 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The entire gene was replaced with a mutated Rbpj cDNA coding for histidine rather than arginine at position 218, a floxed neo cassette, and a poly A tail. Cre mediated recombination removed the neo cassette. This allele expresses a dominant negative form of Rbpj. Whole mount in situ hybridization analysis confirmed the absence of Mesp2 expression in E11.5 homozygous embryos. |
