| Primary Identifier | MGI:4867476 | Allele Type | Targeted |
| Attribute String | Constitutively active, Inserted expressed sequence, Null/knockout | Gene | Mesp2 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The entire gene was replaced with a fusion of the viral coactivator VP16 activation domain and Rbpj, a floxed neo cassette, and a poly A tail. Cre mediated recombination removed the neo cassette. This allele expresses a constitutively active form of Rbpj. Whole mount in situ hybridization analysis confirmed the absence of Mesp2 expression in E11.5 homozygous embryos. |
