| Primary Identifier | MGI:4878876 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Kiss1 |
| Transmission | Germline | Strain of Origin | (C57BL/6J x 129S6/SvEvTac)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A targeting construct was used to insert an IRES-cre-FRT-pgk-neo-FRT cassette into the Kiss1 locus after exon 2. Properly targeted ES cell clones were injected into C57BL/6J blastcysts to produce KissICneo+ chimeras that were backcrossed to C57BL/6J animals. To remove the neo cassette, mice were bred to Flp deleter mice, then backcrossed to C57BL/6J for 6 generations. |
