Primary Identifier | MGI:4881773 | Allele Type | Targeted |
Attribute String | Modified isoform(s), Null/knockout, Reporter | Gene | Triobp |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Nearly all of exon 8 was replaced by a nlacZ reporter cassette and a floxed neo cassette, which was removed by cre-mediated recombination. The absence of isoform 5 transcript and isoform 4/5 proteins in the inner ear was confirmed by RT-PCR and western blot analysis respectively. Isoform 1 is retained. |