| Primary Identifier | MGI:4887599 | Allele Type | Targeted |
| Attribute String | Conditional ready, Hypomorph, Modified isoform(s), Reporter | Gene | Hbs1l |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Special note about mutant cell line EPD0693_2_A02: The mutation generated in this ES cell line results in a hypomorphic allele. RT-PCR analysis revealed a 10% residual expression of mRNA in E9.5 stage homozygous mutant embryos. Western blot analysis confirmed residual expression at the expected band size for HBS1L-V3 in various tissues, with some residual expression of V1/V2 (J:272028). |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 21212311 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 21213197. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
