| First Author | Barbour M | Year | 2016 |
| Journal | Sci Rep | Volume | 6 |
| Pages | 38999 | PubMed ID | 27958388 |
| Mgi Jnum | J:255080 | Mgi Id | MGI:6102002 |
| Doi | 10.1038/srep38999 | Citation | Barbour M, et al. (2016) MAP kinase phosphatase 2 deficient mice develop attenuated experimental autoimmune encephalomyelitis through regulating dendritic cells and T cells. Sci Rep 6:38999 |
| abstractText | Mitogen-activated protein kinase phosphatases (MKPs) play key roles in inflammation and immune mediated diseases. Here we investigated the mechanisms by which MKP-2 modulates central nervous system (CNS) inflammation in experimental autoimmune encephalomyelitis (EAE). Our results show that MKP-2 mRNA levels in the spinal cord and lymphoid organs of EAE mice were increased compared with naive controls, indicating an important role for MKP-2 in EAE development. Indeed, MKP-2(-/-) mice developed reduced EAE severity, associated with diminished CNS immune cell infiltration, decreased proinflammatory cytokine production and reduced frequency of CD4(+) and CD8(+) T cells in spleens and lymph nodes. In addition, MKP-2(-/-) CD11c(+) dendritic cells (DCs) had reduced expression of MHC-II and CD40 compared with MKP-2(+/+) mice. Subsequent experiments revealed that CD4(+) T cells from naive MKP-2(-/-) mice had decreased cell proliferation and IL-2 and IL-17 production relative to wild type controls. Furthermore, co-culture experiments showed that bone marrow derived DCs of MKP-2(-/-) mice had impaired capability in antigen presentation and T cell activation. While MKP-2 also modulates macrophage activation, our study suggests that MKP-2 is essential to the pathogenic response of EAE, and it acts mainly via regulating the important antigen presenting DC function and T cell activation. |
