| Primary Identifier | MGI:4949113 | Allele Type | Targeted |
| Attribute String | Inducible, Inserted expressed sequence, RMCE-ready | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae. |
| molecularNote | RMCE on KH2 cells inserted a cassette that contains a tetracycline responsive element (TRE or tetO) and mouse AP-2gamma (Tcfap2c) cDNA sequence into the 3'UTR. With doxycycline treatment, Tcfap2c expression from the Col1a1 locus is widespread with high levels in skin, intestine and stomach, while lower levels are seen in the heart, spleen, thymus and several other organs. No expression is seen in the brain or testes. |
