| Primary Identifier | MGI:4949132 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Hcrtr2 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed transcription disrupter cassette was inserted into intron 1, 137 bp upstream of exon 2 via homologous recombination. The transcription disrupter cassette includes an engrailed 2 splice acceptor, SV40 enhancer, Neo, and a Myc-associated zinc finger protein-binding site. RT-PCR analysis confirmed the absence of full length transcript and the presence of a mutant mRNA. |
