| Primary Identifier | MGI:4949672 | Allele Type | Targeted |
| Attribute String | Modified isoform(s) | Gene | Ldb3 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 12. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 12. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed exon 12, knocking out the long splice variant. The absence of the long form and expression of the short form was confirmed by western blot analysis. |