| Primary Identifier | MGI:4951216 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Aldob |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-Regeneron |
| description | Cell line 15350B-F10 was successfully used to make chimeric mice. Germline transmission was accomplished. Cell line 15350A-A6 was not successfully used to make chimeric mice. J:220330 |
| molecularNote | The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 8162bp between positions 49536744-49544905 of Chromosome 4 (Build GRCm39). The cassette replaced 3' of exon 2 through 5' of exon 9. Enzyme activity assays of liver lysates revealed that activity toward fructose 1-phosphate was undetectable, and resulted in a change in the fructose 1,6-bisphosphate:fructose 1-phosphate activity ratio from 1.7 in wild-type mice to over 10 in homozygous mutant mice, indicating that any remaining aldolase activity was due to aldolase A. |
