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Allele : Col1a1<tm11(tetO*-RFP/RNAi:Rpa3)Slowe> collagen, type I, alpha 1; targeted mutation 11, Scott Lowe
Primary Identifier  MGI:4999906 Allele Type  Targeted
Attribute String  Inducible, Knockdown, Reporter, RMCE-ready Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
description  The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae.
molecularNote  RMCE on KH2 cells inserted a cassette that contains a modified tetracycline promoter (TREtight or Tt; expression in the absence of doxycycline is 40-fold less than with the standard tetO promoter) and a fast-maturing RFP (turboRFP; tRFP) fusion with an Mir30e-based short hairpin sequence targeting Rpa3 (shRpa3.561) into the 3'UTR.
  • mutations:
  • Insertion
  • synonyms:
  • Col1a1<tm1(TREtight-turboRFP-shRpa3.561)Swl>,
  • shRpa3.561,
  • Col1a1<tm1(TREtight-turboRFP-shRpa3.561)Swl>,
  • shRpa3.561
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1 Feature

Genome

0 Expresses

1 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

4 Publication categories





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