Primary Identifier | MGI:5000228 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Boc |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 540 bp region including the entire first coding exon was deleted and an IRES-hAP and floxed PGK-neo cassette was inserted in exon 2 via homologous recombination. Western blot analysis confirmed the absence of protein expression in homozygous embryos. Alkaline phosphatase activity mirrors endogenous gene expression. |