| First Author | Lin Y | Year | 2021 |
| Journal | Theranostics | Volume | 11 |
| Issue | 1 | Pages | 426-444 |
| PubMed ID | 33391484 | Mgi Jnum | J:340221 |
| Mgi Id | MGI:6800402 | Doi | 10.7150/thno.50281 |
| Citation | Lin Y, et al. (2021) Oscillating lncRNA Platr4 regulates NLRP3 inflammasome to ameliorate nonalcoholic steatohepatitis in mice. Theranostics 11(1):426-444 |
| abstractText | Background: Understanding the molecular events and mechanisms underlying development and progression of nonalcoholic steatohepatitis is essential in an attempt to formulating a specific treatment. Here, we uncover Platr4 as an oscillating and NF-kappaB driven lncRNA that is critical to the pathological conditions in experimental steatohepatitis Methods: RNA-sequencing of liver samples was used to identify differentially expressed lncRNAs. RNA levels were analyzed by qPCR and FISH assays. Proteins were detected by immunoblotting and ELISA. Luciferase reporter, ChIP-sequencing and ChIP assays were used to investigate transcriptional gene regulation. Protein interactions were evaluated by Co-IP experiments. The protein-RNA interactions were studied using FISH, RNA pull-down and RNA immunoprecipitation analyses Results: Cyclic expression of Platr4 is generated by the core clock component Rev-erbalpha via two RevRE elements (i.e., -1354/-1345 and -462/-453 bp). NF-kappaB transcriptionally drives Platr4 through direct binding to two kappaB sites (i.e., -1066/-1056 and -526/-516 bp), potentially accounting for up-regulation of Platr4 in experimental steatohepatitis. Intriguingly, Platr4 serves as a circadian repressor of Nlrp3 inflammasome pathway by inhibiting NF-kappaB-dependent transcription of the inflammasome components Nlrp3 and Asc. Loss of Platr4 down-regulates Nlrp3 inflammasome activity in the liver, blunts its diurnal rhythm, and sensitizes mice to experimental steatohepatitis, whereas overexpression of Platr4 ameliorates the pathological conditions in an Nlrp3-dependent manner. Mechanistically, Platr4 prevents binding of the NF-kappaB/Rxralpha complex to the kappaB sites via a physical interaction, thereby inhibiting the transactivation of Nlrp3 and Asc by NF-kappaB. Conclusions: Platr4 functions to inactivate Nlrp3 inflammasome via intercepting NF-kappaB signaling. This lncRNA might be an attractive target that can be modulated to ameliorate the pathological conditions of steatohepatitis. |
