| Primary Identifier | MGI:5007821 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ifnb1 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted 42 bp upstream of the lone coding exon and a FRT-flanked neomycin cassette, another loxP site, and a myc-tagged luciferase coding sequence were inserted downstream of the exon in the BstAPI restriction site. After targeting in C57BL/6 ES cells, the neomycin cassette was removed by flp-mediated recombination. Cre-mediated recombination removed the coding exon. |
