Primary Identifier | MGI:5008628 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | 129/Sv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The firefly luciferase gene (from the pGL2 plasmid, Promega) was introduced into the ROSA26 locus using a knock-in strategy. The targeting vector was constructed to contain the coding sequence of firefly luciferase downstream of a loxP-flanked STOP cassette (consisting of a promoter-less neomycin gene followed by a three SV40 polyadenylation sequences). |