| Primary Identifier | MGI:5009138 | Allele Type | Spontaneous |
| Gene | Ndrg1 | Strain of Origin | NOD/ShiLtJWehi |
| Is Recombinase | false | Is Wild Type | false |
| description | The mutant phenotype was observed during production of the NOD.B6-Slc9a1b/Gem (Chr 4; J:60311) congenic strain. Fine-mapping analysis employing simple sequence length polymorphisms (SSLPs) differentiating NOD, C57BL/6 and DBA/2 (the second parental strain in the mapping cross) demonstrated that the mutation had occurred in a segment of Chr 15 derived from NOD. (J:198695) |
| molecularNote | More than 5 kilobase pairs of DNA encompassing exons 10-14 have been deleted. Northern blot analysis of kidney RNA reveals the presence in mutant mice of a shorter than normal mRNA, at levels similar to those of the normal transcript in wild-typie littermates; the cDNA sequence confirms that the mutant mRNA has exon 9 spliced in-frame to exon 15. Western blot analysis of protein extracts from sciatic nerve with antibodies to full-length NDRG1 reveals an intense 43-kDa band in wild-type and a very faint band of 32 kDa in mutant sciatic nerve. |
