| Primary Identifier | MGI:5014097 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Otof |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false | Project Collection | Neuroscience Blueprint cre |
| molecularNote | A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an FRT-flanked PGK-Neo cassette into the initiation codon of the otoferlin (Otof) locus. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6 mice to establish the colony. Next, mutant mice were bred with FLPe-expressing mice on a C57BL/6 congenic background to remove the FRT-flanked PGK-Neo cassette. The resulting Otof-CreERT2 mice were then bred to C57BL/6J inbred mice for approximately two generations (selecting away from the FLPe transgene). |
