| First Author | Donaldson PD | Year | 2022 |
| Journal | Adv Healthc Mater | Volume | 11 |
| Issue | 18 | Pages | e2200626 |
| PubMed ID | 35869830 | Mgi Jnum | J:347067 |
| Mgi Id | MGI:7620566 | Doi | 10.1002/adhm.202200626 |
| Citation | Donaldson PD, et al. (2022) Polymer Skulls With Integrated Transparent Electrode Arrays for Cortex-Wide Opto-Electrophysiological Recordings. Adv Healthc Mater 11(18):e2200626 |
| abstractText | Electrophysiology and optical imaging provide complementary neural sensing capabilities - electrophysiological recordings have high temporal resolution, while optical imaging allows recording of genetically-defined populations at high spatial resolution. Combining these two modalities for simultaneous large-scale, multimodal sensing of neural activity across multiple brain regions can be very powerful. Here, transparent, inkjet-printed electrode arrays with outstanding optical and electrical properties are seamlessly integrated with morphologically conformant transparent polymer skulls. Implanted on transgenic mice expressing the Calcium (Ca(2+) ) indicator GCaMP6f in excitatory neurons, these "eSee-Shells" provide a robust opto-electrophysiological interface for over 100 days. eSee-Shells enable simultaneous mesoscale Ca(2+) imaging and electrocorticography (ECoG) acquisition from multiple brain regions covering 45 mm(2) of cortex under anesthesia and in awake animals. The clarity and transparency of eSee-Shells allow recording single-cell Ca(2+) signals directly below the electrodes and interconnects. Simultaneous multimodal measurement of cortical dynamics reveals changes in both ECoG and Ca(2+) signals that depend on the behavioral state. |
