| Primary Identifier | MGI:5086014 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Eno2-Glrb)456Wha |
| Inheritance Mode | Recessive | Strain of Origin | (C57BL/6 x DBA/2)F1 x DBA/2 |
| Is Recombinase | false | Is Wild Type | false |
| description | This is one of four transgenic founder lines that were derived (J:32212). Despite the very low expression of the transgene, when homozygous it rescues the reproductive and much of the neurologic phenotype of Glrbspa/ Glrbspa mice (J:60037). |
| molecularNote | The transgene contains the promoter of the rat enolase 2, gamma neuronal gene driving expression of a cDNA encoding the beta subunit of the rat glycine receptor, which is followed by the SV40 small T-antigen splice and polyadenylation signals. . Brains of both heterozygous and homozygous transgenic mice of line 456 on a homozygous Glrbspa background contain Glrb mRNA and membrane-bound glycine receptor alpha-1 subunit (a proxy for the beta receptor subunit), analyzed via mRNA northern and western blot analysis, respectively, at levels not significantly different from those in non-transgenic Glrbspa homozygotes. Despite this, binding of strychnine to spinal cord membrane preparations from the homozygous transgenic mice is consistently higher than to preps from the non-transgenic animals, though still quite low. |
