| First Author | Shin HJ | Year | 2021 |
| Journal | Aging Cell | Volume | 20 |
| Issue | 7 | Pages | e13410 |
| PubMed ID | 34087956 | Mgi Jnum | J:310503 |
| Mgi Id | MGI:6724177 | Doi | 10.1111/acel.13410 |
| Citation | Shin HJ, et al. (2021) Nucleotide-binding domain and leucine-rich-repeat-containing protein X1 deficiency induces nicotinamide adenine dinucleotide decline, mechanistic target of rapamycin activation, and cellular senescence and accelerates aging lung-like changes. Aging Cell 20(7):e13410 |
| abstractText | Mitochondrial dysfunction has long been implicated to have a causative role in organismal aging. A mitochondrial molecule, nucleotide-binding domain and leucine-rich-repeat-containing protein X1 (NLRX1), represents the only NLR family member that targets this cellular location, implying that NLRX1 probably establishes a fundamental link between mitochondrial functions and cellular physiology. However, the significance of NLRX1 function in cellular senescence, a key conceptual constituent in aging biology, is yet to be defined. Here, we demonstrate that molecular hallmarks involved in aging biology including NAD(+) decline, and activation of mTOR, p53, and p16(INK4A) are significantly enhanced in NLRX1 deficiency in vitro. Mechanistic studies of replicative cellular senescence in the presence or absence of NLRX1 in vitro reveal that NLRX1-deficient fibroblasts fail to maintain optimal NAD(+) /NADH ratio, which instigates the decline of SIRT1 and the activation of mTOR, p16(INK4A) , and p53, leading to the increase in senescence-associated beta-galactosidase (SA-beta-gal)-positive cells. Importantly, the enhanced cellular senescence response in NLRX1 deficiency is significantly attenuated by pharmacological inhibition of mTOR signaling in vitro. Finally, our in vivo murine studies reveal that NLRX1 decreases with age in murine lungs and NLRX1 deficiency in vivo accelerates pulmonary functional and structural changes that recapitulate the findings observed in human aging lungs. In conclusion, the current study provides evidence for NLRX1 as a crucial regulator of cellular senescence and in vivo lung aging. |
