| Primary Identifier | MGI:5284881 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Sema5a |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 6 and an FRT flanked inverted neo cassette and second loxP site were inserted downstream of exon 6 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exon 6. Western blot analysis confirmed the absence of protein expression in homozygous mice. |