| Primary Identifier | MGI:5294414 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Cd8a |
| Transmission | Germline | Strain of Origin | C57BL/6NHsd |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert a cre-recombinase coding sequence, a neomycin resistance cassette, and an internal ribosome entry site (IRES) into the start codon of the CD8 antigen alpha chain (Cd8a) gene. The construct was electroporated into C57BL/6NHsd-derived LK1 embryonic stem (ES) cells. Chimeras were bred to C57BL/6 animals. Offspring were crossed to Flp-expressing mice to delete the neo cassette. |
