| Primary Identifier | MGI:5294796 | Allele Type | Targeted |
| Attribute String | Inducible, Null/knockout, Recombinase | Gene | Lgr5 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | A targeting vector was generated so that a dsRed-IRES-cre/ERT2-pA-Frt-neo-Frt cassette was inserted immediately after the ATG codon in exon 1 of the Lgr5 locus (deleting the remainder of exon 1 and splice donor site in intron 1 - a212 bp deletion) by homologous recombination in C2 ES cells. Correctly targeted clones were transfected with an Flpe plasmid to remove the neo cassette. |
