Primary Identifier | MGI:5297555 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Elp1 |
Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted into intron 19 and a floxed neo-stop cassette was inserted into intron 20 via homologous recombination. Cre mediated recombination removed exon 20 and the neo-stop cassette. RT-PCR analysis confirmed the expression of a stable mRNA lacking exon 20. |