| Primary Identifier | MGI:5297159 | Allele Type | Targeted |
| Attribute String | Inducible, Knockdown, RMCE-ready | Gene | Gt(ROSA)26Sor |
| Transmission | Chimeric | Strain of Origin | Not Specified |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | ES cell lines = C57BL/6 and G4. |
| molecularNote | Recombinase mediated cassette exchange was used to insert two short hairpin RNAi specific for the Brachyury (T) gene under the combined control of tetO and CAG followed by a PGK promoter into the Gt(ROSA)26Sortm1Bgh allele. The tetracycline silencer prevents expression of the shRNAi until mice are treated with doxycycline. |
