| Primary Identifier | MGI:5303387 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Hprt1 |
| Transmission | Cell Line | Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| molecularNote | The Ple230-EGFP plasmid (pEMS1422) was created by inserting the Ple230 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human Thy-1 cell surface antigen (THY1) gene) into the multiple cloning site of the pEMS1306 plasmid vector backbone. This placed the Ple230 MiniPromoter upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an enhanced green fluorescent protein (EGFP; with mutated TAA to TTA stop), a tetracysteine residue, a nuclear localization signal, a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple230-EGFP construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1204 embryonic stem cells. |
