| Primary Identifier | MGI:5303419 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Hprt1 |
| Transmission | Cell Line | Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 |
| Is Recombinase | true | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| molecularNote | The CAG-Cre plasmid (pEMS1293) was created by inserting the 1627 bp CAG promoter (a human cytomegalovirus enhancer, chicken beta-actin promoter and 5' UTR, and chicken beta-actin intron/rabbit beta-globin splice acceptor, all from the pDrive-CAG vector) into the multiple cloning site of the pEMS1312 plasmid vector backbone. This placed the CAG promoter upstream of a full F5 mutant-frt site, a Kozak consensus sequence, a Cre recombinase sequence, a full wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This CAG-Cre construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1202 embryonic stem cells. |
