| Primary Identifier | MGI:5303420 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Hprt1 |
| Transmission | Cell Line | Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 |
| Is Recombinase | true | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| molecularNote | The promoterless-Cre plasmid (pEMS1312) was created by inserting no Pleiades Promoter into the multiple cloning site of the pEMS1312 plasmid vector backbone. Therefore, no promoter is upstream of a full F5 mutant-frt site, a Kozak consensus sequence, a Cre recombinase sequence, a full wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This promoterless-Cre construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1202 embryonic stem cells. |
