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Allele : Col1a1<tm1(tetO-RNAi:Rps19)Karl> collagen, type I, alpha 1; targeted mutation 1, Stefan Karlsson
Primary Identifier  MGI:5304754 Allele Type  Targeted
Attribute String  Inducible, Knockdown Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
description  The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae.
molecularNote  RMCE on KH2 cells inserts an shRNA targeting Rsp19 behind a tetracycline responsive element and CMV promoter replacing the Pou5f1 cassette in Col1a1tm2(tetO-Pou5f1)Jae into the 3'UTR. The level of knock down by this allele following doxycycline treatment is lower compared to mice expressing Col1a1tm2(tetO-RNAi:Rps19)Karl .
  • mutations:
  • Insertion
  • synonyms:
  • shRNA-B,
  • shRNA-B
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1 Feature

Genome

0 Expresses

1 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

6 Publication categories





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