| First Author | Müller HP | Year | 2019 |
| Journal | Transl Neurodegener | Volume | 8 |
| Pages | 27 | PubMed ID | 31485326 |
| Mgi Jnum | J:354567 | Mgi Id | MGI:7735037 |
| Doi | 10.1186/s40035-019-0163-y | Citation | Muller HP, et al. (2019) Longitudinal diffusion tensor magnetic resonance imaging analysis at the cohort level reveals disturbed cortical and callosal microstructure with spared corticospinal tract in the TDP-43 (G298S) ALS mouse model. Transl Neurodegener 8:27 |
| abstractText | BACKGROUND: In vivo diffusion tensor imaging (DTI) of the mouse brain was used to identify TDP-43 associated alterations in a mouse model for amyotrophic lateral sclerosis (ALS). METHODS: Ten mice with TDP-43 (G298S) overexpression under control of the Thy1.2 promoter and 10 wild type (wt) underwent longitudinal DTI scans at 11.7 T, including one baseline and one follow-up scan with an interval of about 5 months. Whole brain-based spatial statistics (WBSS) of DTI-based parameter maps was used to identify longitudinal alterations of TDP-43 (G298S) mice compared to wt at the cohort level. Results were supplemented by tractwise fractional anisotropy statistics (TFAS) and histological evaluation of motor cortex for signs of neuronal loss. RESULTS: Alterations at the cohort level in TDP-43 (G298S) mice were observed cross-sectionally and longitudinally in motor areas M1/M2 and in transcallosal fibers but not in the corticospinal tract. Neuronal loss in layer V of motor cortex was detected in TDP-43 (G298S) at the later (but not at the earlier) timepoint compared to wt. CONCLUSION: DTI mapping of TDP-43 (G298S) mice demonstrated progression in motor areas M1/M2. WBSS and TFAS are useful techniques to localize TDP-43 (G298S) associated alterations over time in this ALS mouse model, as a biological marker. |
