| Primary Identifier | MGI:5310947 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Nsun2 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 69756505 of Chromosome 13 upstream of the critical exon(s) (Build 37). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 69757300. Exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created from Nsun2tm1a(EUCOMM)Wtsi by flp recombinase expression in mice carrying this allele. |
