| First Author | Danglot L | Year | 2012 |
| Journal | J Neurosci | Volume | 32 |
| Issue | 6 | Pages | 1962-8 |
| PubMed ID | 22323709 | Mgi Jnum | J:181320 |
| Mgi Id | MGI:5311042 | Doi | 10.1523/JNEUROSCI.4436-11.2012 |
| Citation | Danglot L, et al. (2012) Absence of TI-VAMP/Vamp7 leads to increased anxiety in mice. J Neurosci 32(6):1962-8 |
| abstractText | Vesicular (v)- and target (t)-SNARE proteins assemble in SNARE complex to mediate membrane fusion. Tetanus neurotoxin-insensitive vesicular-associated membrane protein (TI-VAMP/VAMP7), a vesicular SNARE expressed in several cell types including neurons, was previously shown to play a major role in exocytosis involved in neurite growth in cultured neurons. Here we generated a complete constitutive knock-out by deleting the exon 3 of Vamp7. Loss of TI-VAMP expression did not lead to any striking developmental or neurological defect. Knock-out mice displayed decreased brain weight and increased third ventricle volume. Axon growth appeared normal in cultured knock-out neurons. Behavioral characterization unraveled that TI-VAMP knock-out was associated with increased anxiety. Our results thus suggest compensatory mechanisms allowing the TI-VAMP knock-out mice to fulfill major developmental processes. The phenotypic traits unraveled here further indicate an unexpected role of TI-VAMP-mediated vesicular traffic in anxiety and suggest a role for TI-VAMP in higher brain functions. |
