| First Author | Rigaut G | Year | 1999 |
| Journal | Nat Biotechnol | Volume | 17 |
| Issue | 10 | Pages | 1030-2 |
| PubMed ID | 10504710 | Mgi Jnum | J:181755 |
| Mgi Id | MGI:5314120 | Doi | 10.1038/13732 |
| Citation | Rigaut G, et al. (1999) A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol 17(10):1030-2 |
| abstractText | We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms. |
