| First Author | Kouskoff V | Year | 1995 |
| Journal | J Immunol Methods | Volume | 180 |
| Issue | 2 | Pages | 273-80 |
| PubMed ID | 7714342 | Mgi Jnum | J:99452 |
| Mgi Id | MGI:3582241 | Doi | 10.1016/0022-1759(95)00002-r |
| Citation | Kouskoff V, et al. (1995) Cassette vectors directing expression of T cell receptor genes in transgenic mice. J Immunol Methods 180(2):273-80 |
| abstractText | We describe a pair of cassette vectors that can be used to express rearranged T cell receptor genes in transgenic mice. Short DNA fragments containing rearranged V alpha and V beta segments are readily amplified from T cells and introduced between artificial cloning sites. Transgene-derived mRNAs are transcribed under the control of the natural TCR alpha and -beta promoter/enhancer elements. Using this vector, we have obtained transgenic mouse lines which display transgene-encoded TCR alpha and beta chains on a majority of T cells. |
