| Primary Identifier | MGI:5319631 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(NPHS2-Trpc6)F419Walz |
| Strain of Origin | C57BL/6J x CBA/J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The Trpc6-HA wildtype cDNA sequence was made by modifying a Trpc6 cDNA sequence (encoding a 930 amino acid protein) originally isolated from mouse brain tissue by both repairing an A2615G point mutation (D872G) and also by in-frame addition of an influenza virus hemaglutinin epitope (HA-tag) at the 3' end. The cDNA sequence was placed downstream of a 2.5 kbp fragment from the promoter/enhancer region of the human podocin locus (NPHS2) and upstream from a bovine growth hormone polyA sequence. Line F419 contains 2-4 copies of the transgene. |
