| Primary Identifier | MGI:5320891 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence | Gene | Egfr |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting construct introduced an FRT site flanked neomycin resistance gene cassette into intron 20 and a T->G point mutation in exon 21, changing leucine codon 860 (CTG) to arginine (CGG) (p.L860R). The locus was re-targeted to place a lox-Stop-lox (LSL) cassette in intron 1. Flp-mediated recombination removed the neo cassette. The mutation is the equivalent of the human p.L858R mutation associated with lung cancer susceptibility. |
