| First Author | Berna-Erro A | Year | 2014 |
| Journal | Blood Cells Mol Dis | Volume | 52 |
| Issue | 2-3 | Pages | 108-15 |
| PubMed ID | 24074949 | Mgi Jnum | J:317966 |
| Mgi Id | MGI:6840467 | Doi | 10.1016/j.bcmd.2013.08.007 |
| Citation | Berna-Erro A, et al. (2014) The canonical transient receptor potential 6 (TRPC6) channel is sensitive to extracellular pH in mouse platelets. Blood Cells Mol Dis 52(2-3):108-15 |
| abstractText | The canonical transient receptor potential-6 (TRPC6) is a receptor-activated non-selective Ca(2+) channel regulated by a variety of modulators such as diacylglycerol, Ca(2+)/calmodulin or phosphorylation. The present study is aimed to investigate whether different situations, such as acidic pH, exposure to reactive oxygen species (ROS) or hypoxic-like conditions modulate TRPC6 channel function. Here we show normal aggregation and Ca(2+) mobilization stimulated by thrombin in TRPC6 KO platelets; however, OAG (1-oleoyl-2-acetyl-sn-glycerol)-evoked Ca(2+) entry was attenuated in the absence of TRPC6. Exposure of mouse platelets to acidic pH resulted in abolishment of thrombin-evoked aggregation and attenuated platelet aggregation induced by thapsigargin (TG) or OAG. Both OAG-induced Ca(2+) entry and platelet aggregation were greatly attenuated in cells expressing TRPC6 channels. Exposure of platelets to H2O2 or deferoxamine did not clearly alter thrombin, TG or OAG-induced platelet aggregation. Our results indicate that TRPC6 is sensitive to acidic pH but not to exposure to ROS or hypoxic-like conditions, which might be involved in the pathogenesis of the altered platelet responsiveness to DAG-generating agonists in disorders associated to acidic pH. |
