| Primary Identifier | MGI:5430522 | Allele Type | Targeted |
| Attribute String | Constitutively active, Inducible, Inserted expressed sequence, RMCE-ready | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae. |
| molecularNote | RMCE on KH2 cells, which contain Gt(ROSA)26Sortm1(rtTA*M2)Jae and a FRT-flanked PGK-Neo-pA in the 3' UTR of Col1a1, replaced the FRT-flanked neo cassette with a cassette that contained the tetracycline response element driving inducible expression of the mouse cDNA with nucleotide substitutions that result in the amino acid substitution of alanine for serine at position 127 (S127A). This mutation results in a constitutively active protein. |
