Primary Identifier | MGI:5430219 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Vangl2 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 4. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 4. Cre-mediated recombination removed exon 4 and flp-mediated recombination removed the neo cassette. Western blot analysis confirmed the absence of protein expression in brain lysates. |