| First Author | Colón-Teicher L | Year | 1993 |
| Journal | Nucleic Acids Res | Volume | 21 |
| Issue | 9 | Pages | 2223-8 |
| PubMed ID | 8502564 | Mgi Jnum | J:11894 |
| Mgi Id | MGI:60164 | Doi | 10.1093/nar/21.9.2223 |
| Citation | Colon-Teicher L, et al. (1993) Genomic sequences capable of committing mouse and rat fibroblasts to adipogenesis. Nucleic Acids Res 21(9):2223-8 |
| abstractText | The mouse Swiss 3T3-F442A/3T3-C2 cell system is well suited for the isolation of genes involved in commitment to adipogenesis. 3T3-F442A cells convert to adipocytes with high efficiency in response to confluence and insulin. The sister clonal line 3T3-C2 does not respond to these signals, but can convert to adipocytes when transfected with DNA from 3T3-F442A preadipocytes or from human fat. Human fat-tissue biopsy FO46 DNA transfected into 3T3-C2 gave rise to fat foci after two rounds of transfection and selection. A cosmid library of a subclone of secondary transfectant 3T3-C2/FO46-1 was screened for the human repetitive Alu sequence. Five out of eight Alu+ recombinant clones committed 3T3-C2 cells to adipogenesis. The adipose commitment (AC) activity of one cosmid, p18A4, was found to reside in two small, non-identical, subcloned sequences 1.2kb and 2.0kb in length, each separately able to commit 3T3-C2, precrisis mouse and rat fibroblasts and the multipotential C3H10T1/2 cell line to adipogenesis. We conclude that commitment to adipogenesis can be effected in vitro with high efficiency by transfection of specific sequences into a variety of host cells. |
