| Primary Identifier | MGI:5435875 | Allele Type | Transgenic |
| Attribute String | Inducible, Recombinase | Gene | Tg(ACTA1-cre/Esr1*)2Kesr |
| Strain of Origin | (C57BL/6 x C3H)F2 | Induced With | tamoxifen |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The human alpha-skeletal actin gene promoter (ACTA1; HSA) was cloned into a vector upstream the cre recombinase gene fused at the N- and C-termini to mutated estrogen receptor ligand-binding domains (MerCreMer). A beta-globin intron II sequence followed by a polyadenylation sequence was inserted downstream of the promoter to ensure proper splicing. The construct was injected into B6C3F2 embryos. Five founders were generated and crossed to Rosa-lacz reporter mice to assess germline transmission and inducible muscle-specific recombinase activity. Line 2 was established. |
